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. 2019 Sep 17;10(2):313–326. doi: 10.1016/j.apsb.2019.09.003

Figure 1.

Figure 1

SAM-mediated gene activation in human foreskin fibroblasts. (A) Schematic of the experimental design for selecting efficient sgRNAs. (B) qRT-PCR and Western blotting analysis of endogenous GHMT genes. (C) and (D) Infection with a mixture of sgRNA lentiviruses expressing GATA4, HAND2, MEF2C and TBX5 for 4 days, cells were collected for Western blotting (C) and qRT-PCR analysis (D). Error bars indicate SEM. ***P < 0.01.