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. 2019 Sep 17;10(2):313–326. doi: 10.1016/j.apsb.2019.09.003

Figure 3.

Figure 3

Gradual down-regulation of fibroblast genes along the course of reprogramming. (A) and (B) Representative flow cytometry plots (A) and quantification (B) of FSP1+ and α-SMA+ cells in THY1+ cells, showing a high percentage of cells expressing FSP1 and α-SMA. Isotype-matched normal IgG served as negative controls. (C) qRT-PCR analysis of fibroblast genes after infection with GHMT at the indicated time points. (D) and (E) Representative staining images (D) and quantification (E) of FSP1 and α-SMA at the indicated time points. (F) Western blotting analysis of FSP1 and α-SMA at the indicated time points. Error bars indicate SEM. *P < 0.05, **P < 0.01. Scale bars, 50 μm.