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. 2019 Sep 17;10(2):313–326. doi: 10.1016/j.apsb.2019.09.003

Figure 4.

Figure 4

Induction of CMs, SMCs and ECs during cardiac reprogramming. (A) and (B) Representative staining images (A) and quantification (B) of CM markers such as cTNT and α-actinin, SMC marker such as SM-MHC and EC markers such as CD34 and CD31 after 4 weeks of infection with GHMT. (C) qRT-PCR analysis of cardiac genes MYOCD and TNNT2 at the indicated time points. (D) Immunofluorescence labeling of cTNT, α-actinin and tropomyosin by co-staining with α-SMA after 4 weeks of infection with GHMT. (E) Quantitative data of (D) showing the percentages of cTNT+, α-actinin+ or tropomyosin+ cells that were negative for α-SMA. Error bars indicate SEM. Scale bars, 50 μm (A) and 100 μm (D).