Skip to main content
. 2019 Sep 17;10(2):313–326. doi: 10.1016/j.apsb.2019.09.003

Table 2.

Primers used for quantitative RT-PCR.

Name Sequence (5ʹ–3ʹ)
MESP1 Forward CGAGTCCTGGATGCTCTCTG
MESP1 Reverse ATGAGTCTGGGGACGAGACG
EOMES Forward CATGCAGGGCAACAAAATGTATG
EOMES Reverse GTGTTGTTGTTATTTGCGCCTTTGT
GATA4 Forward AATGCCTGCGGCCTCTACA
GATA4 Reverse AGATTTATTCAGGTTCTTGGGCTTC
HAND2 Forward CCACCAGCTACATCGCCTACCT
HAND2 Reverse TCGTTGCTGCTCACTGTGCTT
MEF2C Forward GAACGTAACAGACAGGTGACAT
MEF2C Reverse CGGCTCGTTGTACTCCGTG
TBX5 Forward AAATGAAACCCAGCATAGGAGCTGGC
TBX5 Reverse ACACTCAGCCTCACATCTTACCCT
MEIS1 Forward GGCACAAGACACGGGACTCA
MEIS1 Reverse CATGGGCTGTCCATCAGGATTA
ISL1 Forward ATCAGGTTGTACGGGATCAAATG
ISL1 Reverse ATGTGATACACCTTGGAGCG
MYOCD Forward AATTTCAGAGGTAACACAGCCTCCA
MYOCD Reverse CGCTTTCAATAAGCACGTCCAG
TNNT2 Forward GCTGTGGCAGAGCATCTATAACTTG
TNNT2 Reverse GCCCGGTGACTTTAGCCTTC
THY1 Forward ATACCAGCAGTTCACCCATCCAGT
THY1 Reverse ATTTGCTGGTGAAGTTGGTTCGGG
FSP1 Forward TCTTGGTTTGATCCTGACTGCT
FSP1 Reverse ACTTGTCACCCTCTTTGCCC
COL1A1 Forward GATTCCCTGGACCTAAAGGTGC
COL1A1 Reverse AGCCTCTCCATCTTTGCCAGCA
COL1A2 Forward CCTGGTGCTAAAGGAGAAAGAGG
COL1A2 Reverse ATCACCACGACTTCCAGCAGGA
GAPDH Forward GCACCGTCAAGGCTGAGAAC
GAPDH Reverse TGGTGAAGACGCCAGTGGA