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. 2020 Feb 11;30(6):1883–1897.e6. doi: 10.1016/j.celrep.2020.01.033

Figure 6.

Figure 6

Phenotypic Analysis of Conditional Gene Expression Knockdown in SMC2PTD and SMC4PTD Transgenic Lines at Various Proliferative Stages during the Life Cycle

(A) Average number of nuclei per schizont (mitotic division within red cell). n = 5 (minimum 100 cells). Error bar, ± SEM.

(B) Number of exflagellation centers (mitotic division during male gametogenesis) per field at 15 min post-activation. n = 3 independent experiments (10 fields per experiment). Error bar, ± SEM.

(C) Percentage ookinete conversion from zygotes. Minimum of 3 independent experiments (minimum 100 cells). Error bar, ± SEM.

(D) Live cell imaging of WTGFP, SMC2PTD, and SMC4PTD oocysts (endomitosis in parasite within mosquito gut) at 9, 14, and 21 days post-infection (dpi), using 10× and 63× magnification to illustrate differences in size and frequency. Scale bar, 5 μm (63×) and 20 μm (10×).

(E) Number of oocysts at 9, 14, and 21 dpi. n = 3 independent experiments with a minimum of 5 mosquito guts. Error bar, ± SEM.

(F) Oocyst diameter at 9, 14, and 21 dpi. n = 3 independent experiments. Error bar, ± SEM.

(G) Number of sporozoites at 14 and 21 dpi in mosquito gut. n = 3 independent experiments with a minimum of 5 mosquito guts. Error bar, ± SEM.

(H) Number of sporozoites at 21 dpi in mosquito salivary gland. Minimum of 3 independent experiments. Error bar, ± SEM.

Unpaired t test was performed for statistical analysis: ∗p < 0.05 ∗∗p < 0.01, and ∗∗∗p < 0.001. See also Figure S5, Table S4, and Videos S1 and S2.