Figure 1.

Small GC Structures Are Formed in PPs of SAP-Deficient Mice

(A) TPLSM images of popliteal LNs derived from AID^Cre/+ Rosa26^{Stop-tdTomato/+} WT, SAP^KO, or TCRα^KO mice 7 days after intra-footpad NP-OVA immunization. One day before imaging, naive CFP B cells were transferred to the mice. Scale bar, 200 μm.

(B and C) TPLSM images of mesenteric LNs (B) and PPs (C). Scale bar, 200 μm.

(D) Number of GC structures per PP in WT, SAP^KO, and TCRα^KO mice.

(E) Quantification of GC area of TPLSM images of the mice as shown in (C).

(F) TPLSM images of PPs derived from AID-GFP WT, SAP^KO, or TCRα^KO mice. Scale bar, 200 μm.

(G) Quantification of GC area from TPLSM images of the mice as shown in (F).

Data are pooled from three independent experiments with two mice in each experiment. Each dot in (D) represents a single PP; each dot in (E) and (G) represents a single GC structure; line represents the mean. ^∗p < 0.05, ^∗∗∗p < 0.0001, one-way ANOVA with Bonferroni posttest. ns, not significant.