Figure 3.

The Proportion of Tfh Cells in PP GCs Is Maintained in SAP-Deficient Mice

(A) Representative flow cytometry plots showing activated Th cell (CD44^hi CD62L^lo) frequencies in the PPs of WT and SAP^KO mice.

(B) Representative flow cytometry plots showing Tfh cells (CXCR5⁺ PD-1⁺) gated from the activated T cell population in the PPs of WT and SAP^KO mice.

(C and D) Graphs showing the frequencies of CD4⁺ activated (C) and Tfh cells (D), as in (A) and (B), respectively.

(E) Representative histogram showing Bcl6 expression in WT and SAP^KO Tfh cells. Expression in naive T cells is shown as a negative control, and frequencies are summarized in the graph.

(F) Graph showing the ratio between GC B cells and Tfh cells in WT and SAP^KO PPs.

(G–I) Representative histogram and quantification of ICOSL (G), CD40 (H), and ICAM-1 (I) expression in PP GC B cells.

(J) Representative flow cytometry plots showing EdU uptake by WT and SAP^KO GC B cells, 2.5 h following EdU administration. Frequencies of EdU⁺ cells are summarized in the graph.

Data are pooled from two independent experiments, with three mice in each experiment. Each dot represents a single mouse; line represents the mean. ^∗p < 0.05, ^∗∗∗p < 0.0001, two-tailed Student’s t test. ns, not significant.