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. 2020 Feb 11;30(6):1910–1922.e5. doi: 10.1016/j.celrep.2020.01.032

Figure 4.

Figure 4

SAP-Mediated T Cell Help Is Not Required for Generation of IgA+ GC B Cells in PPs

(A) Representative flow cytometry plots showing IgG1 and IgA isotype frequency gated from GC B cells derived from WT or SAP-deficient mice.

(B) Quantification of GC isotype frequencies, as shown in (A). Data are pooled from four independent experiments; each dot represents a single mouse; line represents the mean.

(C) Levels of IgG1 in the sera of WT and SAPKO mice as estimated by standard ELISA.

(D) Quantification of IgA antibodies in the intestinal contents of WT and SAPKO mice as measured by ELISA.

(E) qPCR for IL21R, IL4R, STAT3, and STAT6 transcripts in sorted GC B cells (GL7+ FAS+) derived from PPs of WT and SAPKO mice.

Data are pooled from two independent experiments with two mice in each experiment. Each dot represents a single mouse, line represents the mean. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.0001, two-tailed Student’s t test. ns, not significant.