Figure 6.

PC Generation in PPs Is Regulated by SAP in an Indirect Manner

(A) Representative flow cytometry plots and quantification of Blimp-1⁺ CD138⁺ cell population in the PPs of WT and SAP^KO Blimp-1-YFP reporter mice.

(B) Representative flow cytometry plots and quantification of the Blimp-1⁺ Dump⁻ (CD4⁺ CD8⁺ F4/80⁺ Gr-1⁺) cell population in the lamina propria of WT and SAP^KO Blimp-1-YFP reporter mice.

(C) Surface IgA frequency gated from the lamina propria Blimp-1⁺ cells shown in (B).

(D) Representative flow cytometry plots and quantification of CD138⁺ cell population in PPs of WT and SAP^KO mice.

(E) Graph showing the ratio between the frequency of CD138⁺ cells to GC size in PPs of WT and SAP-deficient mice.

In (A)–(C), data are pooled from two independent experiments with three mice in each experiment; line represents the mean. In (D) and (E), data are pooled from five independent experiments, with coupled CD138 and GC staining; line represents the mean. ^∗p < 0.05, ^∗∗∗p < 0.0001, two-tailed Student’s t test. ns, not significant.