Figure 4.

Monocytes are able to be incorporate in the aorta ex vivo, after endothelial Lipopolyssacharides/Lysophosphatidic acid (LPS/LPA)-related injury. Aortas were ex vivo exposed to LPS (0.5 µg/mL) plus LPA (0.5 µg/mL) to induce endothelial dysfunction. Afterwards LPS plus LPA were removed and monocytes were incubated with aortas. (A) Murine aorta section incubated with LPS plus LPA, showing no positive cells for human vWF (bars 10 µm). (B) Murine aorta section incubated with LPS plus LPA followed by incubation with monocytes, showing positive cells for human vWF (bars 10 µm). Nuclei are blue (DAPI).