Figure 4.

Modulation effect of maspin on tumorigenesis of bladder carcinoma HT1376 cells. Four-week-old male athymic nude mice were divided randomly into two groups. HT_shCOL (A) and HT_shMapsin (B) cells (5 × 10⁶) were injected subcutaneously in the dorsal area of the mice (n = 6). The tumor volumes were measured every two to three days during 36-day periods (C). The (D) tumor weight (±SE, n = 6) and (E) protein levels of maspin (n = 3) of the tumor derived from HT_shCOL and HT_shMaspin cells were measured after sacrifice. The numbers indicate the ratio of Maspin/β-Actin in relation to the tumor tissue derived from HT_shCO cells. (F) The ratio of mRNA levels of maspin, cyclin D1, p21, p27, vimentin, MMP2, and MMP9 between tumors derived from HT_shCOL and HT_shMaspin cells was determined through RT-qPCR assays (±SE, n = 6). * p < 0.05, ** p < 0.01.