Figure 7.

CREPT expression influences actin filament/focal adhesion formation-related signaling molecules in BLM cells. (a) Western blotting was performed to evaluate expression of RhoA, Diaphanous related Formin 1 (mDia1), FilaminA, focal adhesion kinase (FAK), Talin-1, Paxillin, Tensin 2. Beta-actin (ACTB) was used as the loading control. Representative results are shown followed by mean protein expression values from four independent experiments. (b) Activated RhoA levels were quantified with small GTPase activation assay, and relative values are shown as mean ± SEM of three independent experiments. ** p < 0.01, *** p < 0.001.