Table 1.
Serial xenotransplantation of NSTS-11 cells in NSG mice. A total of 3 × 105 parental NSTS-11 cells or cultured cells derived from the xenograft tumors obtained in a previous in vivo passage were subcutaneously injected into the mice.
| In Vivo Passage Number | Tumorigenic Efficiency | Mean Tumor Volume Increase (mm3/Day ± SEM) | |
|---|---|---|---|
| Mice with Tumors/Mice Injected | Tumors Formed in Total 1 | ||
| First | 3 of 3 (100%) | 3 | 2.89 ± 0.74 (n = 3) |
| Second | 6 of 9 (67%) | 9 | 4.37 ± 1.83 (n = 9) |
| Third | 27 of 27 (100%) | 33 | 5.66 ± 0.72 (n = 27) * |
1 In several mice, subcutaneously injected cells formed two spatially separated tumors within the site of injection and these tumors were handled separately in subsequent experiments. SEM, standard error of the mean; n, number of mice evaluated. * Statistically significant compared with the first in vivo passage (p < 0.05; Welch’s t-test).