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. 2019 Dec 26;9(1):68. doi: 10.3390/cells9010068

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TDP-43 overexpression induces a decrease in cellular viability measured after 48 h of transfection. (A): Number of alive cells analyzed by trypan blue exclusion (n = 3; * p < 0.05). (B) Cell cycle analysis performed by flow cytometry (n = 4–5; * p < 0.05). (C) Percentage of sub-G1 events (fragmented nuclei) analyzed by flow cytometry (n = 4–5; * p < 0.05). (D) Cell viability analyzed by MTT reduction (n = 4; * p < 0.05). (E) LDH released in the medium (n = 4; * p < 0.05). (F) Percentage of positive cells for PI fluorescence (n = 5–6; * p < 0.05). (G) Microscopic analysis of cells transfected with increasing concentrations of TDP-43 and incubated with PI (scale bar = 200 µm).