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. 2019 Dec 18;12(1):3. doi: 10.3390/cancers12010003

Figure 2.

Figure 2

Zip14 is induced in cachectic muscles in PDAC models and is associated with elevated zinc levels. (A) Results from qRT-PCR analysis of Zip14 in the gastrocnemius (Gast.) muscles from Pan02 (left) and FC1242 (right) mice (Tb, blue bars) compared to controls (Con, gray bars) and Pan02 and FC1242 cell lines (Tum, white bars). (B) Results from metal ion analysis of zinc (Zn2+), iron (Fe2+), manganese (Mn2+), and copper (Cu2+) on gastrocnemius muscles from Pan02 (top) and FC1242 (bottom) models compared to control mice at endpoint. Results are depicted as micrograms (µg) of metal ion per gram (g) of muscle dry weight. (C) Results from qRT-PCR analysis of Mt1 and Mt2 in the gastrocnemius muscles from Pan02 (top) and FC1242 (bottom) mice (Tb, blue bars) compared to controls (Con, gray bars). n = 3–5 mice/group. Data are represented as the mean ± SEM. p-values for qRT-PCR analysis and metal ion analysis were determined by using the two-tailed, unpaired Student’s t-test and Mann–Whitney test, respectively. Con: Control; Tb: Tumor-bearing; Tum: Tumor cell lines; Gast.: Gastrocnemius muscle.