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. 2020 Jan 4;12(1):131. doi: 10.3390/cancers12010131

Figure 8.

Figure 8

The effect of Gin Rd on TRPM7 knockout MDA-MB-231 cell biology. (A) Viability. Gin Rd (100 µM) suppressed viability in WT-231 only, while Gin Rd (200–400 µM) significantly suppressed viability in both WT-231 and KO-231, with a higher suppression rate in WT-231 than KO-231. Gin Rd (200 µM) was used in the subsequent experiments. (B–E) show data for cells exposed to 200 µM Gin Rd for 24 h. (B) LDH release. Gin Rd had no significant effect on LDH. (C) Apoptosis. Gin Rd slightly induced apoptosis in both cell lines. (D) Cell cycle analysis. The knock-out of TRPM7 did not affect the cell cycle. Gin Rd caused an increase in the percentage of cells in the S phase and a corresponding decrease in the G0/G1 phase in WT-231 but not in KO-231. (E) Representative images from the cell cycle assay of MDA-MB-231. Significant differences (p < 0.05) from control are indicated by “*”.