Figure 2.

Medullospheres (MS) assay. Representative images of MS obtained with MB cell lines (A). MS quantitative analysis: Number (B), area (C). The fraction of wells without MS plotted against cell numbers per wells (LDA, D). Protein expression and relative densitometry of CD133 (E) Gene expression of NANOG (F) and OCT4 in basal conditions; DAOY expression levels are taken as 1. Data are shown as a mean of three biological replicates ± SEM. Differences were tested with Student’s t-test. * p < 0.05, ** p < 0.001; *** p < 0.0001. Bars: 200 μm.