Table 2.

Primers used for this study. The amplification conditions were the same for both the first mPCR (sea to see and ser) and second mPCR (seg to selj and ser), except the annealing temperature (respectively 55 °C and 52 °C): initial denaturation 94 °C for 3 min; 35 cycles of denaturation (94 °C for 30 s), annealing (30 s), extension (72 °C for 90 s); final extension 72 °C for 7 min.

Gene	Name Primer	Sequence (5′-3′)	Product Size (bp)	Reference
sea	GSEAR-1	GGT TAT CAA TGT GCG GGT GG	102	[53]
	GSEAR-2	CGG CAC TTT TTT CTC TTC GG
seb	GSEBR-1	GTA TGG TGG TGT AAC TGA GC	164	[53]
	GSEBR-2	CCA AAT AGT GAC GAG TTA GG
sec	GSECR-1	AGA TGA AGT AGT TGA TGT GTA TGG	451	[53]
	GSECR-2	CAC ACT TTT AGA ATC AAC CG
sed	GSEDR-1	CCA ATA ATA GGA GAA AAT AAA AG	278	[53]
	GSEDR-2	ATT GGT ATT TTT TTT CGT TC
see	SA-U	TGT ATG TAT GGA GGT GTA AC	213	[53]
	SA-E rev	GCC AAA GCT GTC TGA G
ser	SER 1	AGA TGT GTT TGG AAT ACC CTA T	123	[53]
	SER 2	CTA TCA GCT GTG GAG TGC AT
seg	SEG-F	GTT AGA GGA GGT TTT ATG	198	[54]
	SEG-R	TTC CTT CAA CAG GTG GAG A
seh	SEH-F	CAA CTG CTG ATT TAG CTC AG	173	[54]
	SEH-R	CCC AAA CAT TAG CAC CA
sei	SEI-F	GGC CAC TTT ATC AGG ACA	328	[54]
	SEI-R	AAC TTA CAG GCA GTC CA
selj	SEJ-F	GTT CTG GTG GTA AAC CA	131	[54]
	SEJ-R	GCG GAA CAA CAG TTC TGA
sep	SEP-F	TCA AAA GAC ACC GCC AA	396	[54]
	SEP-R	ATT GTC CTT GAG CAC CA