Table 1.
Information of candidate reference gene and qPCR amplification characteristics.
| Gene Symbol | Gene Name | Primer Sequence (5’-3’) | Amplicon Size (bp) | Correlation Coefficient |
|---|---|---|---|---|
| EF-1α | Elongation factor -1α gene | CGTCTGCTTAGTGAACCTGCTC/ GAAGGAGCGAATGTCACAACCA |
112 | 0.996 |
| HIS | Histone | AGGGAAAGCCGCTCCACTTC/ TCGTAACCCTCTTGGCGTGAAT |
152 | 0.996 |
| IF-4α | Eukaryotic initiation factor 4 alpha | CAAGCCGAGCCTGCGATCAT/ TCGTGTTCTGGTCCATGTCTCC |
121 | 0.996 |
| PP2A | Protein phosphatase 2A | AAGCCGAGCCTTCCATCATAGC/ ACCACCACCACCACTCATTGC |
197 | 0.992 |
| TUB | Tubulin gene | GCGTCTTGTCCGTGAGATTGC/ GCCGAGATGAGGTGGTTAAGGT |
109 | 0.994 |
| UBC | Ubiquitin C gene | GCCATCACTCAGAGCGTCATCT/ AAGGAGACTGTGTAGGACCAAGAA |
100 | 0.999 |
| UBQ | Polyubiquitin 10 gene | TGCGTCTACCACTTCAGGATGT/ TCTGCTGGTCTGGAGGAATGC |
145 | 0.992 |
| ACTIN | Actin gene | ATCGTCTGTGACAACGGTAC/ ATGGAGGGTGATGAGATTCAGC |
267 | 0.992 |