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. 2019 Dec 31;12(1):100. doi: 10.3390/cancers12010100

Figure 4.

Figure 4

Detection and confirmation of CSF1 partner genes identified in conventional TGCTs. A partial sequence chromatogram is shown from each fusion transcript, with the arrow depicting the fusion breakpoint. Gel electrophoresis images display respective cDNA fragment amplification. A positive band can be seen in Lane 1, supporting the presence of the fusion product. M, DNA marker (Promega, Madison, WI, USA); Lane 1, patient case; Lane 2, HapMap normal RNA control; Lane 3, no template control (water control). Identification and validation of CSF1-KCNMA1 (A) and CSF1-FN1 (B) fusion transcripts by AMP, Sanger sequencing, and RT-PCR.