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. 2019 Dec 18;12(1):5. doi: 10.3390/cancers12010005

Figure 4.

Figure 4

Poly (ADP-ribose) polymerase (PARP) inhibition augments ultraviolet B (UVB)-mediated mitochondrial fusion. (A) Effect of UVB irradiation and PARP inhibition on mitochondrial morphological subtypes (tubular, intermediate, and fragmented) are quantified based on confocal microscopic images 24 h post-UVB (minimum 29 cells). (B) Mitochondrial morphology visualized by confocal microscopy with Mitotracker Red CMXRos dye (n = 3). Enlarged pictures are displayed at the right bottom corner. Scale bar is presented on the images. (C) The branching aspect of mitochondria was derived from confocal microscopic images and was represented as a form factor (n = 3). (D) Protein expression of mitofusin-1 (Mfn1), mitofusin-2 (Mfn2), and optic atrophy 1 (OPA1) was visualized by Western blot. (E) Expression of mitochondrial fusion proteins were analyzed by Western blot 24 h post-UVB (n = min. 3). −/+ represent vehicle (−) or ABT-888 (+) treatment. *; ** and *** indicate statistically significant difference at p < 0.05 and p < 0.01, p < 0.001, respectively. Error bars represent SEM.