Table 1.

Clinical features of the CNS patients and liquid biopsy source. BRAF V600E analysis by PCR in germline DNA.

Patient ID	Sex	Age	Pathological Diagnosis	Liquid Biopsy Source			Germline DNA
				Serum	Plasma	CSF
LI.3	M	5	Medulloblastoma SHH Subtype	+	+	+	SN
LI.4	F	7	Anaplasic Medulloblastoma	+	+	+	WT
LI.6	F	17	Unresectable Thalamic Anaplastic Glioma	+	+	+	WT
LI.10	M	3	Thalamic Diffuse Astrocytoma	+	+	+	WT
LI.11	F	17	Unresectable Thalamic Pilocytic Astrocytoma	+	+	+	WT
LI.15	M	7	DIPG	+	+		WT
LI.17	M	6	Ependymoma	+	+		WT
LI.18	F	15	DIPG		+	+	WT
LI.21	M	6	Diffuse Glioma	+	+	+	WT
LI.22	F	2	Pilomyxoid Midline Astrocytoma	+	+	+	WT
LI.23	M	8	DIPG	+	+		WT
LI.25	M	8	Pleomorphic Xanthoastrocytoma	+	+		WT
LI.26	F	15	Oligodendroglioma	+	+	+	WT
LI.28	M	7	DIPG	+	+		WT
LI.29	-	9	DIPG	+	+		WT
LI.30	M	12	Thalamic Diffuse Glioma	+	+	+	WT
LI.31	M	2	Cerebellar Ganglioglioma	+	+		WT
LI.33	M	10	DIPG	+	+		WT
LI.34	F	4	DIPG	+	+		WT
LI.35	F	6	DIPG	+	+		WT
LI.36	M	6	Pilomyxoid Thalamic Astrocytoma	+	+		WT
LI.37	M	18	Classic Medulloblastoma	+	+	+	WT
LI.38	M	11	Pilocytic Astrocytoma	+	+		WT
LI.39	M	3	Supratentorial PNET	+	+		SN
LI.40	M	15	Pilocytic Astrocytoma	+	+		WT
LI.41	M	14	GBM	+	+		WT
LI.42	M	1	GBM	+	+	+	WT
LI.43	M	12	Diffuse Midline Glioma	+	+	+	WT
LI.44	F	21	Pilocytic Astrocytoma	+	+	+	WT

SN: Sample Not Available. WT: Wild Type.