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. 2020 Jan 17;12(1):228. doi: 10.3390/cancers12010228

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A1 — Full-length gel images of western blotting against herpes simplex virus thymidine kinase (HSV-tk) which were cropped and presented in Figure 1A. (A) Images from 40 representative plaques (among a total of 120 plaques) harvested after homologous recombination, each expressing a different length of HSV-tk, indicated by 1–3 major bands that were detected from each plaque. (B) Comparison of relative sizes of the three bands from two plaques (among a total of 120 plaques) harvested after homologous recombination (plaque #1: lane 2–4, plaque #2: lane 5–7) to the wild-type HSV (lane 1) and the marker (far left). (C) Viral replication and cytotoxicity of three viruses (OTS-412, OTS-C1, and OTS-C2) in NCI-H460 cancer cell line. Cells were seeded at 1.5 × 10⁴ cells/well, virus concentration was 0.1 PFU/cell, incubation time was 48 h. NT, negative control.