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. 2020 Jan 9;9(1):170. doi: 10.3390/cells9010170

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RK-33 is a broad-spectrum inhibitor of infectious virus. Vero cells were infected with (A) dengue virus serotype 2 (DENV-2), (B) Zika virus (ZIKV), (C) West Nile virus (WNV), (D) respiratory syncytial virus (RSV) or (E) human parainfluenza virus type 3 (hPIV-3) at a multiplicity of infection (MOI) of 1 for 2 h, after which virus was removed, and fresh medium containing 2% FBS was supplemented with the indicated concentration of RK-33. Released virus samples (for hPIV-3, DENV-2, ZIKV and WNV) or cell-associated samples (for RSV) were collected 22 h later, and viral titres (expressed as plaque forming units (pfu) per mL) determined by plaque assay. Dose-response curves were plotted from the viral titres (pfu) versus the logarithmic value of the concentrations of RK-33. Results represent the mean +/− standard deviation (SD) for duplicate wells from a single assay, representative of two (n = 2) independent experiments. See Table 1 for pooled data from the two assays.