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. 2020 Jan 4;9(1):122. doi: 10.3390/cells9010122

Figure 7.

Figure 7

Tre may relieve CS-induced apoptosis and inflammation may through TFEB activation. (A,B) Immunoblotting analysis of BAX and BCL-2 protein levels in lungs on day 7 or 56 post CS instillation (n = 5). (C,D) Immunoblotting analysis of BAX and BCL-2 protein levels in AMs on day 7 or 56 post CS instillation (n = 3). (E–H) ELISA analysis of IL-6, MCP-1, TNF-α, and IL-1β levels in BALF on day 7 or 56 post CS instillation (n = 5 to 6). (I) H&E staining of mouse lungs on day 7 or 56 post-CS instillation (scale bar = 100 μm and n = 5). (J,K) Immunoblotting analysis of BAX and BCL-2 protein levels in MH-S cell lines (n = 3). (L,M) TUNEL (green) and DAPI (blue) staining and ratios of TUNEL-positive apoptotic MH-S cells (scale bar = 50 μm and n = 3). (N–Q) ELISA analysis of IL-6, MCP-1, TNF-α, and IL-1β levels in MH-S cell supernatant (n = 3). Data are presented as the mean ± SD. *, p < 0.05 and **, p < 0.01.