Table 4.

Mouse models of HCC generated via hydrodynamics-based transfection.

Modulation System	Target Genes	Tumor Type	Mouse Strain	Latency	References
Sleeping Beauty transposon	c-Myc	HB	WT FVB/N	~6 weeks	[82]
	Spry2Y55F + ΔN90 β-catenin	HCC	WT FVB/N	~6 months	[88]
	hMet + β-catenin (S33Y or S45Y)	HCC	WT FVB/N	~6 weeks	[86]
	c-Myc + shP53	HCC	WT C57BL/6	~7 weeks	[83]
	YAPS127A + ΔN90 β-catenin	HB	WT FVB/N	~6 weeks	[91]
	myr-Akt + Fbxw7ΔF	ICCA	WT FVB/N	~6 weeks	[90]
	Smad7 + HRASG12V + shP53	HCC	WT C57BL/6	~5 weeks	[89]
	myr-Akt + YAPS127A	ICCA	WT FVB/N	~3 weeks	[91]
	myr-Akt + cMet	HCC	WT FVB/N	~4 weeks	[92]
	RasV12 + ΔN90 β-catenin	HCC	WT FVB/N	~4 weeks	[93]
CRISPR/Cas9	sgPten + sgP53 + Cas9	CK19-positive liver tumors	WT FVB/N	~3 months	[95]
	sgDnajb1 + sgPrkaca + Cas9	FL-HCC	WT FVB/N	~14 months	[96]

HB, hepatoblastoma; FL-HCC, fibrolamellar hepatocellular carcinoma.