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. 2020 Jan 14;9(1):205. doi: 10.3390/cells9010205

Figure 1.

Figure 1

The effect of the co-culturing of cancer cells and fibroblasts on the expression of Hk2 and Ldha. (A) and (B): the co-culturing of fibroblasts (murine lung fibroblasts (MLF) or human lung fibroblasts (HLF)) with respective cancer cells (KLN205 or A549) decreased the level of Hk2 in cancer cells, having only a minor effect on the enzyme in fibroblasts. (C) and (D): in the co-cultures, the expression of Ldha was reduced in cancer cells, but was elevated in fibroblasts. Bar = 50 µm. The black and white images depict differences in the shape and size of fibroblasts and cancer cells in the co-cultures. The ratio of the fluorescence of Hk2 and Ldha to β-actin in the MLF and HLF monocultures was assumed to be 1. Asterisks indicate a statistically significant difference (p < 0.05). All the experiments were performed in triplicate and obtained similar results, and representative data from one experiment are shown in the figure.