Table 1.
Alfalfa small RNA (sRNA) sequencing datasets. Statistics of sRNA sequences for CK (normal water), PEG (polyethylene glycol-6000) drought stress, and PEG + SNP (sodium nitroprusside; drought stress and SNP treatment) libraries from Medicago sativa L. ‘‘Sandeli’’. Percentage refers to the net read code, which is equal to the ratio of each category to raw reads. rRNA: filtering ribosomal RNA, snoRNA: nucleolar small RNA, tRNA: transport RNA.
| Category | CK | PEG | PEG + SNP | |||
|---|---|---|---|---|---|---|
| Count | Percentage | Count | Percentage | Count | Percentage | |
| Raw reads | 26,849,326 | 100.00% | 24,252,135 | 100.00% | 20,514,941 | 100.00% |
| Clean reads | 23,528,741 | 87.63% | 18,046,004 | 74.41% | 15,076,692 | 73.49% |
| Mapped to genome | 10,366,134 | 38.61% | 7,651,367 | 31.55% | 6,487,853 | 31.63% |
| miRNAs | 1,267,908 | 4.72% | 1,180,088 | 4.87% | 871,935 | 4.25% |
| rRNA | 2,931,163 | 10.92% | 3,527,200 | 14.54% | 1,974,535 | 9.62% |
| snoRNA | 3746 | 0.01% | 7001 | 0.03% | 10,497 | 0.05% |
| tRNA | 234,634 | 0.87% | 333,859 | 1.38% | 417,378 | 2.03% |
| Without annotation | 20,273,991 | 75.51% | 14,113,193 | 58.19% | 12,602,624 | 61.43% |