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. 2020 Feb 7;8(1):106–123. doi: 10.1002/iid3.289

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© 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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graphic file with name IID3-8-106-g005.jpg — CD169 and CD64 flow cytometry expressions on natural or incubated blood. First, leukocytes were isolated from red blood cells or debris or apoptotic cells based on their typical side (SS INT) and forward scatter (FS INT). Then, CD169 and CD64 were respectively measured on the surface of the monocytes (CD169 on monocytes) and of the neutrophils (CD64 on neutrophils). Examples of their expressions were given for three subjects: A, One first healthy volunteer whole blood. B, The same healthy volunteer whole blood that was not activated by interferons (IFNs), but that staid 15 hours at 37°C. C, The same healthy volunteer whole blood that was activated by IFNα1 for 15 hours at 37°C. D, The same healthy volunteer whole blood that was activated by IFNγ for 15 hours at 37°C. E, One‐second viral‐infected whole blood, and (F) one‐third bacterial‐infected whole blood

graphic file with name IID3-8-106-g006.jpg — CD169 and CD64 flow cytometry expressions on natural or incubated blood. First, leukocytes were isolated from red blood cells or debris or apoptotic cells based on their typical side (SS INT) and forward scatter (FS INT). Then, CD169 and CD64 were respectively measured on the surface of the monocytes (CD169 on monocytes) and of the neutrophils (CD64 on neutrophils). Examples of their expressions were given for three subjects: A, One first healthy volunteer whole blood. B, The same healthy volunteer whole blood that was not activated by interferons (IFNs), but that staid 15 hours at 37°C. C, The same healthy volunteer whole blood that was activated by IFNα1 for 15 hours at 37°C. D, The same healthy volunteer whole blood that was activated by IFNγ for 15 hours at 37°C. E, One‐second viral‐infected whole blood, and (F) one‐third bacterial‐infected whole blood