Table 1.
Sequences of primers used for real-time PCR.
| Gene Name | RefSeq Accession Number | Primer | Amplicon Size | Tm | Primer Sequence (5′-3′) Forward |
|---|---|---|---|---|---|
| leptin | NM_000230.3 | Fw | 150 | 60.0 | GGCTTTGGCCCTATCTTTTC |
| Rv | 60.0 | CCAAACCGGTGACTTTCTGT | |||
| IL1β | NM_000576.3 | Fw | 205 | 60.0 | GGGCCTCAAGGAAAAGAATC |
| Rv | 60.0 | TTCTGCTTGAGAGGTGCTGA | |||
| IL6 | NM_000600.5 | Fw | 180 | 59.8 | AGGAGACTTGCCTGGTGAAA |
| Rv | 59.8 | CAGGGGTGGTTATTGCATCT | |||
| IL8 | NM_000584.4 | Fw | 227 | 60.0 | TAGCAAAATTGAGGCCAAGG |
| Rv | 60.0 | AAACCAAGGCACAGTGGAAC | |||
| IL17 | NM_002190.3 | Fw | 216 | 60.0 | CCCCAGTTGATTGGAAGAAA |
| Rv | 60.0 | GAGGACCTTTTGGGATTGGT | |||
| CXCL5 | NM_002994.5 | Fw | 218 | 60.0 | GACGGTGGAAACAAGGAAAA |
| Rv | 59.9 | GCTTAAGCGGCAAACATAGG | |||
| MIF | NM_002415.2 | Fw | 234 | 59.6 | AGAACCGCTCCTACAGCAAG |
| Rv | 59.8 | ATTTCTCCCCACCAGAAGGT | |||
| VEGF | NM_001025366.3 | Fw | 186 | 60.0 | CCCACTGAGGAGTCCAACAT |
| Rv | 60.0 | TTTCTTGCGCTTTCGTTTTT | |||
| TNF-α | NM_000594.4 | Fw | 173 | 60.1 | TCCTTCAGACACCCTCAACC |
| Rv | 60.0 | AGGCCCCAGTTTGAATTCTT | |||
| IFNɣ | NM_000619.3 | Fw | 198 | 59.6 | TCCCATGGGTTGTGTGTTTA |
| Rv | 59.7 | AAGCACCAGGCATGAAATCT | |||
| TET1 | NM_030625.3 | Fw | 173 | 59.8 | AGGTCCAGGGCCAAATAACT |
| Rv | 59.9 | AGAAGGTGCCAGGTCAGAGA | |||
| β-actin | NG_007992.1 | Fw | 209 | 59.9 | AGAAAATCTGGCACCACACC |
| Rv | 59.9 | AACGGCAGAAGAGAGAACCA | |||
| β-actin | NG_007992.1 | Fw | 352 | 60.0 | AAACTGGAACGGTGAAGGTG |
| Rv | 59.9 | CTCAAGTTGGGGGACAAAAA | |||
| β-actin | NG_007992.1 | Fw | 479 | 59.9 | AAGATGACCCAGGTGAGTGG |
| Rv | 59.9 | GGGGTGTTGAAGGTCTCAAA | |||
| β-actin | NG_007992.1 | Fw | 638 | 59.9 | CTCTTCCAGCCTTCCTTCCT |
| Rv | 60.0 | AAAGCCATGCCAATCTCATC | |||
| β-actin | NG_007992.1 | Fw | 718 | 59.0 | CTCTTCCAGCCTTCCTTCCT |
| Rv | 60.0 | CACCTTCACCGTTCCAGTTT | |||
| FABP4 | NM_001442.3 | Fw | 181 | 60.0 | TACTGGGCCAGGAATTTGAC |
| Rv | 60.0 | GTGGAAGTGACGCCTTTCAT | |||
| AdipoQ | NM_001177800.2 | Fw | 173 | 60.0 | CCTAAGGGAGACATCGGTGA |
| Rv | 60.0 | GTAAAGCGAATGGGCATGTT | |||
| PDK1 (+ve) | NC_000002.12 | Fw | 100 | 62.0 | CGCCCTGTCCTTGAGCC |
| Rv | 62.9 | CGGTATGGAGCGTCCCCT | |||
| PDK1 (-ve) | NC_000002.12 | Fw | 486 | 56.5 | CTGATAAGACTACACTGGACG3 |
| Rv | 56.6 | CCCAAGATACTACACTACCATC |
PDK1 (+ve) and PDK1 (-ve) are positive and negative controls for HIF1α ChIP assay, respectively.