Figure 1.

Expression of GS1 in the retina rescues neuronal death induced by Htt-Q93. (A) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of GS1 mRNA expression in larvae from control w¹¹¹⁸ of animals expressing the EP-GS1^G4337 (GS1) line using the actin-Gal4 driver. The relative level of GS1-mRNA is reported as arbitrary units compared to actin5C used as a control. At least three separate experiments were performed in duplicate. (B) Western blot from larvae extracts showing the relative amount of GS1 protein from w¹¹¹⁸ (CTL) animals or expressing GS1. Tubulin was used as the loading control. (C) GS1 enzymatic activity in extracts from whole larvae used in (A,B). *** p < 0.001, **** p < 0.0001 in panels (A,C) were calculated from Student’s t-test from at least three independent experiments.(D–G)) Photographs of Drosophila-compound eyes (lateral view) from females at 20 days after eclosion (DAE) expressing the indicated UAS-transgenes using the GMR-Gal4 driver. (H–K) Representative photographs of the retina from animals of the indicated genotype. (L) Fluorescent image of a fly head expressing UAS-GFP under the GMR-Gal4 promoter (sagittal view), bar 100 μm. (M) Quantification of GFP from photographs of adult eyes at 20 DAE of the indicated genotypes. The asterisks represent the p-values from One-way analysis of variance (ANOVA) with Tukey multiple comparison * p < 0.05 and **** p < 0.0001, and the error bars indicate the standard deviations.