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. 2020 Jan 4;9(1):123. doi: 10.3390/cells9010123

Figure 11.

Figure 11

EVs from ZIKV-infected C6/36 modify towards a pro-coagulant, pro-inflammatory, and pro-adherent phenotype and favor permeability in naïve endothelial cells (ECs). (A) EC TF-1+ percentages (FACS) at different EVs stimuli conditions from three independent experiments. (B) EC Protease Activated Receptor+ (PAR-1) percentages at different EVs stimuli conditions from three independent experiments. (C) EC intercellular adhesion molecule-1+ (ICAM-1) percentages at different EVs stimuli conditions from three independent experiments. The TF, PAR-1, or ICAM-1 levels were compared (by an unpaired Student’s t-test) between all conditions’ values. (D) TNF-α mRNA expression (RT-PCR) in naïve ECs at different EVs stimuli conditions. The TNF-α genome conserved region (amplicon of 600 bp) was visualized on 2% ethidium bromide-stained 1.2% agarose gel. (E) Permeability percentages obtained by assessing the fluorescein isothiocyanate (FITC)-Dextran pass through the EC monolayers in the presence of different EV stimuli conditions. Three independent experiments were performed. For the 100% FITC-Dextran delivered control, a no-cell insert was used. The endothelial vascular permeability percentages were compared (by an unpaired Student’s t-test) between all conditions’ values. For all experiments, statistical significance was recognized as *, +, ¡, #, !,/, ~, or ° when p < 0.05, **, ++, ¡¡, ##, !!,//, ~~, or °° when p < 0.01, and ***, +++, ¡¡¡, ###, !!!,///, ~~~, or °°° when p < 0.0001.