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Downregulation of USP4 in stemness-enriched lung cancer cells. (A) Top panels: Stemness of mouse D121, LLC, and human H460, HCC827, and H1299 lung cancer cell lines was enriched by sphere formation. Photos show sphere cells of each cell line. (B) Bottom panels: Expression of stemness-associated genes in parental D121 and LLC cells and the corresponding sphere cells analyzed by RT-qPCR. Data presented as mean ± SD of three independent experiments. ** P < 0.01.
