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. 2020 Jan 8;12(1):148. doi: 10.3390/cancers12010148

Figure 7.

Figure 7

Stable USP4 knockdown increases the stemness, transforming ability, and therapeutic resistance of lung cancer cells. (A) Expression of stemness-associated genes in control and USP4 knockdown lung cancer cells analyzed by RT-qPCR. (B) Control and USP4 knockdown D121 and H1299 cells were cultured in defined serum-free medium for 2 weeks to allow sphere formation. (C) Sphere formation compared between control D121 cells and D121 cells with stable USP4 knockdown cultured in defined serum-free medium and treated with/without 1 μM BMS345541(NF-κB inhibitor) for 2 weeks. (D) Control and USP4 knockdown lung cancer cells were treated with the indicated concentration of cisplatin or doxorubicin for 48 h and cell viability measured by MTS assay. (E) Control and USP4 knockdown lung cancer cells were treated with/without 1 μM BMS345541 for 24 h. Cell surface expression of PD-L1 as analyzed by flow cytometry. Left panels: Representative histograms. Right panels: Quantitation. Data presented as mean ± SD of three independent experiments. * P < 0.05; ** P < 0.01.