Figure 5.

CBP/p300-binding domain of E1Aad5 is responsible for stabilization of FoxO1 protein. (a) Wild-type E1A 12S (wt) and Rb-binding mutant E1A 12S (YH47) induce FoxO1 protein accumulation in NIH3T3 cells. NIH3T3 cells were transfected with the adenovirus E1A constructs (E1A12S (wt), E1A12S (RG2), or E1A12S (YH47)). Cell extracts were analyzed by immunoblotting. (b) E1A + Ras cells were transfected with cytomegalovirus (CMV)-p300-hemagglutinin (HA) and treated with NaBut for 24 h. Cell extracts were immunoprecipitated with anti-HA antibody, followed by immunoblotting with FoxO1 antibody. (c) Cell extracts from E1A + Ras cells were immunoprecipitated with anti-acetylated lysine antibody and probed with anti-FoxO1 antibody.