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. 2020 Jan 19;9(1):249. doi: 10.3390/cells9010249

Figure 5.

Figure 5

Chronic HFD up-regulates the amyloidogenic pathway in HFD mouse brain. (a) Represents the expression level of beta-amyloid-cleaving enzyme (BACE-1), amyloid precursor protein (APP), APP-C-terminal fragment βeta (APP-CTFβ) and amyloid beta (Aβ) proteins via western blot. β-actin was used as a loading control. (b) Given are the representative images of Immunofluorescence staining of Aβ in the cortex and hippocampal (CA1 and DG region) of the HFD mice brain (n = 12 mice/group) (c) Thioflavin S staining representing the sign of early plaque deposition in the cortex and hippocampus region of control and HFD mice brain. (d) Western blot analysis indicating expression level of BACE-1 and APP proteins in control and AdipoR1 knockdown in embryonic mouse hippocampal cell line mHippoE-14 (AdipoR1 shRNA). Data are presented as mean ± SEM. Data for immunofluorescence and western experiment are compared using the Student’s t-test and other data are compared using one-way ANOVAs. Data are compared using U\the unpaired Student’s t-test. Significance = * p < 0.05, ** p < 0.01. n.s= non-Significance.