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. 2019 Dec 23;12(1):54. doi: 10.3390/cancers12010054

Figure 1.

Figure 1

Doublecortin-like kinase 1 (DCLK1) surface expression in human colorectal cancer cell lines. HT29, human colorectal cancer (CRC) cells were grown in adherent 2D tissue culture plates and as 3D spheroids. These cells were disassociated and subjected to Fluorescence-activated cell sorting (FACS) using humanized DCLK1 mAb (hCBT-15). HT29 cells grown in 2D culture demonstrated extracellular DCLK1 expression on 9.89% of cells (A—Top panel). HT29 cells grown in 3D matrices demonstrated extracellular DCLK1 expression on 45% of cells (A—bottom panel). The quantitative expression is represented in the bar graph (B). (C,D): HCT116, grown in 2D demonstrated DCLK1 extracellular expression on 20% and 3D demonstrated DCLK1 extracellular on 19.5% of cells. (E,F): LoVo, grown in 2D demonstrated DCLK1 extracellular expression on 22% of cells and 3D demonstrated DCLK1 extracellular on 25% of cells.