Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 23;12(1):54. doi: 10.3390/cancers12010054

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Expression of chimeric antigen receptors (CARs) in Doublecortin-like kinase 1 (DCLK1) CAR-T cells (CBT-511). (A) The illustration represents the structure of DCLK1-CAR (CBT-511). The second-generation CAR with DCLK1 ScFv, CD28 co-stimulatory domain, and CD-3zeta activation domain was generated. (B) Expression of DCLK1-CAR in T cells transduced with lentiviral DCLK1-CAR by Fluorescence-activated cell sorting (FACS) with F(ab)2 antibody. CBT-511 cells were effectively transduced with DCLK1-CAR and expression of CAR (~20%) was confirmed by FACS with F(ab)2 antibody.