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. 2019 Dec 26;11(1):29. doi: 10.3390/genes11010029

Figure 5.

Figure 5

Inhibition of MIR221HG promotes bovine adipocyte differentiation. (A) Interference efficiencies of MIR221HG-specific short interfering RNAs (siRNAs). siRNAs were transfected into preadipocytes. Forty-eight hours after transfection, total RNA was isolated and the expression levels of MIR221HG were detected by qRT-PCR. (B,C) siRNA1-mediated downregulation of MIR221HG promotes the expression of adipogenic markers peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), and fatty acid binding protein 4 (FABP4) at both mRNA (B) and protein (C) levels. The data are presented as mean ± SE from three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.