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. 2020 Jan 18;11(1):111. doi: 10.3390/genes11010111

Figure 2.

Figure 2

Loss of DEPP induction upon HIF1A and HIF2A knockdown in ARPE-19 cells. HIF1A and HIF2A (a), DEPP and ADM (c), transcript levels in normoxia (N) and hypoxia (H) were determined by real-time PCR in presence of HIF1A siRNA (HIF1A KD), HIF2A siRNA (HIF2A KD), or both HIF1A and HIF2A siRNAs (HIFs KD). Data are shown as fold changes over normoxic (N) siCTRL (Ctrl). (b) HIF1A and HIF2A protein levels in cells treated with siRNAs as indicated. Beta Actin (ACTB) served as loading control. Normoxic U87 neuroblastoma cells were used as positive control for HIF1A and HIF2A. Abbreviations see Figure 1. Shown are individual values and means ± SD of N = 3. Statistics: One-way ANOVA with Brown–Forsythe test, *: p ≤ 0.05, **: p ≤ 0.005, ***: p ≤ 0.005, ****: p ≤ 0.0005.