Figure 5.

PKM2 was an alternative pathway under the deficiency of Hsp90 function and Akt phosphorylation. Data represent the means ± SD for three independent experiments. (A) After treatment with triciribine of different concentrations for 24 h, CCK-8 tests were used to detect cell viability. (B) After treatment with triciribine of 50 µm for 14 h, cells were exposed to heat stress for 5 h or not for detecting LPO and MDA levels. (C) After treatment with triciribine of 50 µm and/or 0.1 µM GA for 14 h, cells were exposed to heat stress for 5 h or not. Western blot analysis was used to detect the levels of different proteins with the indicated antibodies. Representative bands are presented in the left panel. Reactive bands were quantified using the Quantity One software, and the relative abundance of the tested proteins was normalized to that of β-actin (right panel). The differences of the data of cells with different treatment vs. that of the Con are indicated by * p < 0.05 and ** p < 0.01. The differences of the data of cells treated by TR + GA + HS vs. that in other groups are indicated by # p < 0.05 and ## p < 0.01. The differences of the data of cells in HS vs. that in other groups are indicated by & p < 0.05 and && p < 0.01.