Figure 3.

OG inhibits the tumor growth of Panc 02 cell grafts in mice. (A) Measurement of the superficial tumor volumes from the C57BL/6 mice subcutaneously inoculated with Panc 02 cell grafts and treated with OG or control 40% PEG400 (CTRL). The measurement was started on Day 14 post-inoculation and continued every other day using Vernier caliper with the formula 1/2 × length × width². (B) Tumors removed from the mice described in (A) on Day 30 post-inoculation. OG treatment significantly inhibited the tumor growth of Panc 02 cell grafts. (C) Representative H&E-stained tumor tissue sections of control and OG-treated mice. OG treatment significantly resulted in loosened cell arrangement and decreased chromatin basophilia. (D) Representative DAPI-stained tumor tissue sections of control and OG-treated mice. OG treatment significantly caused chromatin condensation and nuclear fragmentation. (E) Food intake of the mice was monitored every day. (F) Body weights of the mice were also recorded daily. Data are the mean ± SEM of 6 mice.