Skip to main content
. 2020 Jan 17;12(1):235. doi: 10.3390/cancers12010235

Figure 4.

Figure 4

PAR5 suppresses cell migration of ATC cell lines. (A) Representative images of migration assay performed in FRO and 8505c cells stably transfected with PAR5 or the corresponding EV (left panel). Magnification 40×. Migrating cells were fixed with crystal violet solution. After elution from transwell, it was quantified by reading its absorbance at 590 nm. Crystal violet absorbance values were divided by corresponding cell titer values. In the right panel data obtained from three independent experiments carried out in FRO and 8505c cells are shown. Values are reported as mean value ± SD, compared to the EV, set equal to 1. Unpaired t-test: **, p < 0.01 (PAR5 vs. EV, in FRO cells), ****, p < 0.0001 (PAR5 vs. EV, in 8505c cells). (B,C) A representative image of scratch wound-healing assay performed in FRO and 8505c cells stably carrying PAR5 or the corresponding EV is reported in the upper panel. t = 0 h and t = 24 h post wounding. Magnification 40×. In the lower panel is reported the percentages of closures of the wound after 24 h in FRO and 8505c cells stably expressing PAR5 or carrying the corresponding EV. Data are presented as the average of three experiments. *, p < 0.05 in FRO cell line, PAR5 vs. EV, after 24 h, **, p < 0.01 in 8505c cell line after 24 h.