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. 2020 Jan 6;9(1):133. doi: 10.3390/cells9010133

Figure 9.

Figure 9

Cell viability and octamer-binding transcription factor 4 (Oct4) protein expression were detected in primary cortical progenitor cultures and brain sections. (A) Cell viability and cell number of neuron progenitor cell cultures from TCTP-deficient mice and littermate controls at E15.5 were detected by MTT assay. (*** p < 0.001, Student’s t test, n = 3 per group). (B) TCTP and Oct4 expression was detected by immunohistochemistry with antibody followed by DAB staining and then counterstaining with hematoxylin. (C,D) Oct4 expression of control and TCTP-cKO mice at E13.5 and P0.5 was detected by immunohistochemistry. A representative picture is shown for two independent experiments. (E) Bar graph summarizing quantification of Oct4-positive (+) cells in (D), (Data presented as mean ± SEM, * p < 0.05, ** p < 0.01, Student’s t test, n = 3 per group). (F) Protein level of Oct4 in control and TCTP-cKO mice was measured by Western blotting. Scale bar: A, 40 μm; C: a, d, 400 μm, b, c, e, f, 100 μm; D: a, b, d, e, 100 μm, c, f, 40 μm.