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. 2019 Dec 4;214(2):409–418. doi: 10.1534/genetics.119.302947

Figure 2.

Figure 2

FSHR-1 functions in the nervous system to regulate the UPRmt. (A and B) Left: representative images of posterior intestines of young adult animals or fshr-1 mutants expressing the Phsp-6::GFP transgene following treatment with M9 (control) or PQ for 24 hr. “fshr-1 neuronal rescue” denotes fshr-1 mutants expressing fshr-1 cDNA under the neuron-specific promoter rab-3 (A) or rgef-1 (B). “fshr-1 intestine rescue” denotes fshr-1 mutants expressing fshr-1 cDNA under the intestine-specific promoter ges-1. Right: average GFP fluorescence intensity is quantified. (C) Left: representative images of intestines of wt or fshr-1 mutants expressing Phsp-6::GFP following e.v. control or atfs-1 RNAi treatment by feeding. Right: average GFP fluorescence intensity is quantified. (D) Left: representative images of intestines of wt worms expressing Phsp-6::GFP following e.v. control or fshr-1 RNAi treatment by feeding in the absence or presence of PQ. Right: average GFP fluorescence intensity is quantified. (E) Left: representative images of Phsp-6::GFP fluorescence in intestines of wt or fshr-1 mutants in the absence or presence of transgene uthIs375[unc-119p::cco-1 HP + rol-6(su1006)]). Right: average GFP fluorescence intensity is quantified. (F) Left: representative images of intestinal cells of wt or fshr-1 mutants expressing the mitochondrial marker TOMM-20::mCherry under the intestinal-specific promoter ges-1. Right: average mCherry fluorescence intensities are quantified. (G) Left: representative images of animals expressing soluble GFP in the intestine (Pges-1::GFP) in wt or fshr-1 mutants. Right: average GFP fluorescence intensities are quantified. Bar in the panel represents 10 µm. Error bars indicate ± SEM. One-way ANOVA with Bonferroni post-test ***P < 0.001; #P < 0.001 compared to fshr-1; Prab-3::fshr-1; @P > 0.05 compared to wt; $P > 0.05 compared to wt; and &P > 0.05 compared to wt + PQ. cDNA, complementary DNA; e.v., empty vector; ns, not statistically significant; PQ, paraquat; RNAi, RNA interference; UPRmt, mitochondrial unfolded protein response; wt, wild-type.