Figure 6.

WormCat analysis of RNA-seq data from C. elegans treated with combinations of lifespan-lengthening drugs reveals the emergence of sterol metabolism in drug combinations, limiting survival. (A) Comparison of Metabolism: lipid: sterol enrichment in single, double, and triple-drug combinations shows sterol emergence in the Rifa/Rapa/Psora gene set (Admasu et al. 2018). (B) Diagram showing a summary of data from lifespan changes after triple-drug treatment from Admasu et al. (2018). Pink box denotes drug combination that causes premature death. Bubble heat plot key is the same as Figure 1D. Allan, Allantoin; Psora, Psora-4; Rapa, Rapamycin; Rifa, Rifampicin