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Plasmid replication during transfer under selection. The transferred single strand enters 5′ end first, and a complementary strand is synthesized from multiple primer sequences that form gapped duplex intermediates. A double-strand break creates ends that can recombine with recipient plasmids by homologous recombination and initiate recombination-dependent replication (Kuzminov and Stahl 1999; Malkova and Ira 2013).
