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. 2019 Dec 28;9(1):81. doi: 10.3390/cells9010081

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of astrocyte activation by GFAP immunohistochemistry after 6 weeks of cuprizone treatment (a) Representative images of control (CTRL) and cuprizone-treated (CU) groups in GFAP-Cre^−/− TRPA1^Fl/Fl, GFAP-Cre^+/− TRPA1^Fl/− and GFAP-Cre^+/− TRPA1^Fl/Fl mice.; (b) Pixel intensities of diaminobenzidine staining in the middle part of the corpus callosum in each group; *p < 0.05, n = 3–5/group, Scale bar = 100 µm.