Figure 2.

Effects of daidzein, daidzein-4′-sulfate and equol on neuroglobin intracellular levels in T47D cells. Western blot (left) and densitometric analyses (right) of NGB protein levels in T47D cells treated for 24 h with the vehicle (DMSO), E2 (10 nM), D (1 μM), D4S (1 μM), or Eq (1 μM). The amount of proteins was normalized by comparison with tubulin levels. Data are the mean ± SD of three different experiments. p < 0.001 was determined with Student t-test with respect to the vehicle (*) treated samples. DMSO: dimethyl sulfoxide; E2: estradiol; NGB: neuroglobin; D: daidzein; D4S: daidzein-4′-sulfate; Eq: equol.