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. 2020 Jan 9;12(1):167. doi: 10.3390/cancers12010167

Figure 4.

Figure 4

Daidzein, daidzein-4′-sulfate and equol action mechanism. The phosphorylation of the Ser473 residue of AKT (pAKT) (a,b) and Thr180/Tyr182 residues on P-38 (c,d) was determined by western blot analysis in MCF-7 cells exposed for 1 h (a,c) and 24 h (b,d) to either vehicle (DMSO) or E2 (10 nM) in presence or absence of D, D4S and Eq (1 μM). The nitrocellulose was stripped and then and then probed with anti-AKT or anti-P38 antibodies. In the panels, the pAKT/AKT (a,b) and pP38/P38 (c,d) ratios are represented. These ratios are calculated with respect to tubulin obtained by densitometric analyses of three different experiments (mean ± SD). p < 0.001 was determined by Student t-test with respect to vehicle (*) or Endox-untreated (°) samples. AKT: protein kinase B; E2: estradiol; Endox: endoxifen; ERα: estrogen receptor α; NGB: neuroglobin; p38: p38 mitogen-activated protein kinase; D: daidzein; D4S: daidzein-4′-sulfate; Eq: equol.